I would like to monitor the presence of USB devices and have found modules such as PyUSB that serve this purpose. However, I don't know how to run USB detection services alongside the Tkinter main loop. Is this possible?
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Before starting tk mainloop, start some separate thread that will monitor USBs. Or you can try using tk alarms http://www.pythonware.com/library/tkinter/introduction/x9507-alarm-handlers-and-other.htm to periodically check USB.
Might I suggest that instead of trying to constantly monitor the presence of a USB device you include a search or refresh button that will check just once.