Using this script
df <- data.frame(x = 1:5, y = 1:5, color = letters[1:5])
ggplot(df, aes(x, y, fill = color)) +
geom_point(size = 4, pch = 21) +
guides(fill = guide_legend(
title = expression(atop(Median~Nitrate-Nitrogen~(NO[3]^{textstyle("-")}-N), ".\n Concentration"~(mg~L^{textstyle("-")})))))
I got this figure
Any suggestions how to align the second line of the legend title to the left?