The aim is to convert a pure red image into any hue of the color wheel.
- A monochrome image is first converted into a RGB red image, ex:
- then converted into HSV
- The hue component is modified by adding an angle value supposed to match the wheel color
- then the hsv image is back converted into the rgb color space.
The problem is that only green or blue image can be obtained (no yellow for an angle~30° for example):
The code performed in some ipython cells relies on scikit-image 0.10dev:
from skimage import io
from skimage import color
from scipy import ndimage as nd
import numpy as np
from matplotlib import pyplot as plt
import os
cy55 = io.imread('/home/jeanpat/MFISH/PSI/P07/01/Cy5/P070109C.tif')
zero = np.zeros(cy55.shape,dtype=np.uint8)
rgb0 = np.dstack([cy55, zero,zero])
hue_rotations = [18, 36,72,90,108]
images = {}
images[0] = rgb0
hsv0 = color.rgb2hsv(rgb0)
print hsv0[:,:,0].dtype
for hue in hue_rotations:
hsv = np.copy(hsv0)
hsv[:,:,0] = hsv[:,:,0]+ hue
rgb = color.hsv2rgb(hsv)
images[hue] = rgb
i = 1
plt.figure(num=None, figsize=(15, 5), facecolor='w', edgecolor='k')
for hue in np.sort(images.keys()):
plt.subplot(1,6,i,xticks=[],yticks=[])
plt.title('hue='+str(hue))
plt.imshow(images[hue])
i = i +1
plt.show()